Beyond removing nitrogen and phosphorous from secondary effluent, the����APP settleable microalgae can also synthesize and accumulate such high value-added polymers as lipids, proteins, polysaccharides, poly-β-hydroxybutyrate (PHB), etc.The previous experiments efficiently enriched settleable microalgae with the settleable rate of more than 97% by applying “washout” pressure, and accumulated the highest lipids content of 49.2% (dry cell weight) by changing environmental factors like CO2, light/dark shift, etc. According to the following experiment results, increasing the light intensity and CO2intake would not further increase the lipids content of the settleable microalgae. Instead, the lipids content decreased. However, settleable cyanobacteria had a good ability to synthesize and accumulate PHB. As long as there was a tiny specific carbon source (acetate) stimulation, cyanobacteria could easily synthesize and accumulate PHB with the content of up to 30% without CO2input.Clearly, synthesizing PHB mainly from inorganic carbon source was more attractive than that from organic carbon source by activated sludge.
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